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Immunostaining detection and flow cytometry for transcriptional factor marker in age-dependent surgical NP tissue and 3D construct (days 28). A: staining in surgical tissue; B: Flow cytometry. Representative histograms of flow cytometry at left illustrate the relative fluorescence intensity of NP markers on X-axis for isolated cells via tissue incubation (Brachyury T). C: staining in 3D construct. Bar=50μm.
