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. 2017 Feb 22;4:17004. doi: 10.1038/hortres.2017.4

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The two-phase experimental design intorduced in P. zonale breeding: P1, cultivation of stock plants for obtaining the SCC in location 1; P2, the rooting of stem cuttings to test the root formation in location 2. In P1, and α-design in 2013/14 and row-column design in 2014/15, were used. Each cultivation table represented on replicate having 500 planting positions arranged either in 167 incomplete blocks with three experimental units (EU1) each in 2013/14 or, in year 204/15 in 84 rows and six columns. On each EU1 a pair of stock plants of a genotype was placed in P1. In P2, the total experimental space represented by m rooting tables (at maximum 9) was divided into four regions to which the replicates were systematically assigned. Regions shaded in gray in rooting tables in P2 correspond to replicates shaded in gray of cultivation tables in P1. Eeach rooting table held 36 trays at maximum. One tray contained 39 paper pots arranged in three rows. The trays were divided into areas, representing an experimental unit in P2 (EU2), to which different genotypes were randomly allocated. The size of areas varied depending on the numbers of stem cuttings for a genotype. The planting of stem cuttings followed a row-wise order.