FIGURE 2.

Effects of repeated ith. CORM-2 administration (20 μg/5 μl, ith. 16 and 1 h before CCI and then once daily for 7 days) on the development of mechanical (B; von Frey’s test) and thermal (C; cold plate test) hypersensitivity on day 7 after CCI as measured 2 h after the last drug administration (A). The behavioral results are presented as the mean ± SEM (7–10 rats per group), and the horizontal dotted line shows the cut-off value (B: 26 g, C: 30 s). The Western blot analysis (D–I) shows the influence of chronic ith. CORM-2 administration on protein levels of the P2X4R (D,E), IBA-1 (F,G) and GFAP (H,I) in the spinal cord (D,F,H) and dorsal root ganglia (DRG) (E,G,I) on day 7 after CCI 6 h after the last drug administration. The representative bands are shown below each column of respective group on the graph and come from the same membrane photo. Samples from different groups were not next to each other so were cut from different locations and set together. Biochemical results are presented as the mean ± SEM (4–7 rats per group). The inter-group differences were analyzed using one-way ANOVA with Bonferroni’s multiple comparisons test. ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001 compared to the INTACT animals; ^#p < 0.05; ^##p < 0.01; and ^###p < 0.001 compared to the V-treated CCI group.