Fig. 3.

Scap siRNA KD adds to simvastatin LDL-C lowering in NFR-CETP mice. Mice were dosed orally daily with 100 mg/kg simvastatin or vehicle starting on day 0. On day 4, mice were dosed with nt control-1 siRNA, Pcsk9 siRNA (0.5 mg/kg), and m-Scap-1 siRNA at the indicated doses (milligrams per kilogram). Plasma and livers were collected 10 days later. Liver Scap mRNA (A), liver Hmgcr mRNA (B), plasma PCSK9 (C), and plasma LDL-C (D). Each bar in (A) and (B) represents mean ± SEM. Box and whiskers in (C) and (D) represent the 25th percentile, the median, the 75th percentile (boxes), and the minimum and maximum (whiskers); n = 8 per group. *P < 0.05, **P < 0.01, and ***P < 0.001.