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. 2017 Feb 22;12(2):e0171471. doi: 10.1371/journal.pone.0171471

Fig 6. P. theridion and K. thyrsites detection in the tissues through Twort’s Gram staining.

Fig 6

A) Cluster of P. theridion (small, dark blue organisms) inside myocardial cells in the spongy layer (arrow). Bar scale: 20μm. B) P. theridion in myocardial cells (arrows) and in an endocardial cell (arrowhead). Scale bar: 10μm. C) K. thyrsites inducing nodular granulomatous myositis in red muscle. The parasites are located in both red (star) and white (pound) portion of the skeletal muscle. One plasmodium inside a white muscle cell is also inducing a nodular granulomatous inflammatory reaction (arrow). Scale bar: 250μm. D) Inset of Fig C. Dark blue parasites are visible inside the nodular inflammation sites: 100μm.