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. 2017 Feb 22;12(2):e0172391. doi: 10.1371/journal.pone.0172391

Fig 2. RT-QuIC sodium chloride titration for TME-infected and uninfected cattle brain samples.

Fig 2

RT-QuIC reactions were seeded with 10−4 (A, B) and 10−6 dilution (C, D) of TME-infected and uninfected cattle brain homogenates using a range of NaCl concentrations (100–500 mM) with the full-length bPrP wild type (aa 25–241) as substrate. Data are presented as mean ThT fluorescence of 8 reactions conducted as 2 repeats of 4 reactions. The positive threshold was calculate as ~10,000 relative fluorescence units of normal cattle brain homogenates.