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. 2017 Feb 22;12(2):e0172529. doi: 10.1371/journal.pone.0172529

Fig 4. Vpu fractionates with the membrane fraction and inclusion bodies dependent on time post-induction with IPTG.

Fig 4

The βDDM extractable fraction increases with time until it reaches saturation around 6 hr (A). When the non-aqueous fraction is first treated with urea and then βDDM (B), the protein first fractionates in the detergent fraction, implying that protein does integrate in the membranes that is extractable by βDDM. Vpu is indicated by a black arrow.