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. 2017 Feb 22;12(2):e0172529. doi: 10.1371/journal.pone.0172529

Fig 7. Size-exclusion chromatography of concentrated metal-affinity chromatography-purified PelB-VPU.

Fig 7

Eluate from Fig 6 was subjected to SEC-FPLC. Sec resin: Superdex 200 10/300 GL; Flow-rate: 0.5 mL/min, Running buffer: 20 mM HEPES, pH 7.5, 250 mM NaCl, 5% glycerol and 0.02% βDDM. The minor peak at 8 mL corresponded to the void volume, and the major peak at 11.48 mL contained PelB-Vpu. The column was calibrated with cytochrome c (A), carbonic anhydrase (B), albumin (C), alcohol dehydrogenase (D), β-amylase (E) and blue dextran (F) and a standard curve was obtained as shown in the inset. The size of the Pel-B-Vpu- βDDM complex was estimated to be 315 kDa.