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. 2017 Feb 22;12(2):e0172115. doi: 10.1371/journal.pone.0172115

Fig 2. Changes in predicted targeting gene for miR-155 in HCC827GR cells.

Fig 2

(A) miR-155 targeting predictions were queried in three target prediction databases. (B) Result of the analysis of 172 predicted targets for miR-155. (C) Assessment of luciferase reporter activity in HEK293 cells co-transfected with expression vector of miR-155 and reporter vector of smad2 3’UTR or mutation smad2 3’UTR. The data represent the relative expression levels of renilla luciferase expression standardized to firefly luciferase (***p<0.001 vs. psiCHECK2 smad2 3’UTR miR-NC). (D) The mRNA expression level of smad2 was quantified by qRT-PCR in HCC827GR cells compared to HCC827 cells (***p<0.001 vs. HCC827 cells). (E) Change in protein levels of smad2 in HCC827GR cells compared to HCC827 cells. Results are shown as the ratio of the density of smad2 to that of GAPDH. Each column represents the mean±S.E.M. of 3 independent experiments (***p<0.001 vs. HCC827 cells). (F) Change in protein levels of phospho-smad2 in HCC827GR cells. Results are shown as the mean±S.E.M. of 3 independent experiments (***p<0.001 vs. HCC827 cells).