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. 2017 Feb 22;12(2):e0172358. doi: 10.1371/journal.pone.0172358

Fig 1. Melting curves and amplification plots of 16S rDNA amplicons resulting from PCR using U1/U8 primers.

Fig 1

Melting curves obtained using p_m16S(0.9kb), a plasmid containing internally deleted 16S rDNA from M. capricolum subsp. capricolum strain California Kid (gi_83283139) (a), their reproducibility over multiple quantifications (b), with the amplification plot (c) and the linear regression analysis of Cq as a function of DNA copy input number (d, efficacy Em16S = 1.99847). For (c) and (d), dilutions were done from a freshly prepared 0.9 kb PCR amplicon obtained from 5 pg of p_m16S(0.9kb) using running conditions depicted in Tables 2 and 5 with DNA concentration measured by NanoDrop™ (http://www.nanodrop.com/).