Table 1. Indicator cell lines for mycoplasma detection using indirect Hoechst’ staining.
| Name | Species | Culture medium1 | cells/well2 |
|---|---|---|---|
| MeWo [6] | Human | RPMI 1640 Glutamax-I, 10% fetal calf serum, 1% Sodium pyruvate, 1% non-essential amino acids, 10 mM HEPES pH7.2 | 4x104 |
| Vero [7] | Monkey | DMEM GlutaMax-I,10% fetal calf serum | 8x103 |
| IgH-2 [8] | Iguana | EMEM, 2 mM Glutamax, 10% fetal calf serum 1% non-essential amino acids | 6x103 |
| NIH3T3 [9] | Mouse | RPMI 1640 Glutamax-I, 5% fetal calf serum | 4x103 |
| CHO [10] | Hamster | Ham’s F-12 Nutrient Mixture, 10% fetal calf serum | 4x103 |
1 in humidified incubator, in the presence of 5% CO2 at 37°C
2 amount of cells to be seeded in 2 mL of culture medium in 6 well-plates.