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| Master Mix GFP | Final Concentration | Volume per reaction tube (μL) |
|---|---|---|
| Sterile nuclease-free water (pH 7/8) | 4.6 | |
| FastStart SYBR Green Master (ROX) 2X (Roche, cat.no. 4913850001) | 1X | 10 |
| Primer GFP_for (20 μM) | 0.2 μM | 0.2 |
| Primer GFP_rev (20 μM) | 0.2 μM | 0.2 |
(see Table 2 for primer sequences)
