Table 5. Run program steps for the detection of 16S rDNA of mollicutes adapted from [5].
Cycle Number | Step | Temperature | Period | |
---|---|---|---|---|
1 cycle | Activation of the enzyme | 95°C | 10 min | |
40 cycles | Denaturation | 95°C | 15 s | |
Hybridation / Elongation | 65°C | 2 min | Data collection |