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. 2017 Feb 22;12(2):e0172358. doi: 10.1371/journal.pone.0172358

Table 5. Run program steps for the detection of 16S rDNA of mollicutes adapted from [5].

Cycle Number Step Temperature Period
1 cycle Activation of the enzyme 95°C 10 min
40 cycles Denaturation 95°C 15 s
Hybridation / Elongation 65°C 2 min Data collection