Table 7. Comparison of the sensitivity of five mycoplasma detection assays.
MeWo cells were inoculated with either culture medium (Medium) or 1.5 or 15 CFU of Acholeplasma laidlawii (A.l.). Cell free supernatants were collected after 5, 8 or 12 days of culture and tested with the various assays (see also Fig 4 for details).
| Seeding at day 0 | Days in culture | MycoAlert™ | PlasmoTest™ | Hoechst | PCR | qPCR | |
|---|---|---|---|---|---|---|---|
| Detection | Quantification | ||||||
| Medium | 5 | N | N | N | N | N | NQ |
| 8 | N | N | N | N | N | NQ | |
| A.l. 1.5 CFU | 5 | N | N | N | N | Y | NQ |
| 8 | N | N | N | N | Y | NQ | |
| 12 | N | N | Y | N | Y | NQ | |
| A.l. 15 CFU | 5 | N | N | Y | Trace? | Y | NQ |
| 8 | N | N | Y | Trace? | Y | NQ | |
| 12 | N | N | Y | Y | Y | 1,29 x 105 | |