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. 2017 Feb 22;12(2):e0172677. doi: 10.1371/journal.pone.0172677

Fig 4. Insulin enhances neuronal survival.

Fig 4

(A) Low magnification confocal images of Nissl staining in cultures at 8 DIV, conditions are indicated on the left side of images, scale bars, 500 μm. Chart shows effect of insulin on slice area; (B) Left: neuron counts in CA3c, CA3b and CA1; Right: neuron counts corrected by the normalized slice area. n = 3 cultures, each condition. Statistical differences are indicated for comparison between 1X group and other groups. (C) Representative confocal images of Nissl staining in CA3c, CA3b and CA1, scale bars, 50 μm. (D) Left: representative confocal images of neurons in CA3c, scale bars represent a distance of 5 μm; Right: corresponding cell size in CA3c. n = 30 cells, each condition. Significant statistical differences are indicated for comparison of 0X group versus 1X group. Error bars indicate SD. Significant differences are labeled as *p < 0.05, **p < 0.01 and ***p < 0.001.