Fig 7. Modification of glucose and electrolyte concentrations affects cell survival and ictal activity.

(A-D) Left: brightfield images of cultures maintained in media with indicated concentrations of glucose, Ca²⁺, Mg²⁺, or K⁺ at 14 DIV, scale bars, 500 μm; Right: time course of LDH and lactate release into culture medium. n = 3 cultures, each condition. Error bars indicate SD. Concentrations of ions that correspond to rat CSF are underlined and indicated by solid symbols. Significant statistical differences are indicated for comparison of 4 mM Ca²⁺, 4 mM Mg²⁺ and 8.5 mM K⁺ versus CSF concentrations of corresponding ions., with **p < 0.01 and ***p < 0.001.