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. 2017 Feb 22;12(2):e0172722. doi: 10.1371/journal.pone.0172722

Fig 5. Bioactivity of RGD, Folate and Protamine functional groups attached through click reactions to the FH NP surface.

Fig 5

(a) Flow cytometry histograms for the reaction of RGD-FH (14) and a control NP (b) at three NP concentrations (18 nM, 65 nM, and 155 nM) with BT-20 cells are shown. NP concentrations were determined from iron concentrations using the manufacture’s value of 5874 Fe’s per NP (AMAG Pharmaceuticals Package insert.) Relative fluorescence (c) from (a) and (b) versus NP concentration is shown. Relative fluorescence is the mean fluorescence of (a) minus the mean fluorescence of (b) divided by the fluorescence of unstained cells. (d) Uptake of the 89Zr-Folate-NP (89Zr-11) and a 89Zr-FH control NP is shown in at three NP concentrations. (e) Uptake was blocked by 1 μM folate (10) and was highly temperature dependent (f). (g) Uptake of 89Zr-Cy5.5-Protamine-NP (89Zr-16) at three different NP concentrations is shown. (h) 89Zr-Cy5.5-Protamine-FH (89Zr-16) uptake at 4°C and 37°C is shown (10 nM NPs were used). MDA-MB-231 cells were used for (d) through (h).