Table 1. List of proteins identified using MALDI analysis.
| Spot | Protein | Mass | pI | Score | Matched Peptides | Total Peptides | Protein Sequence Coverage | Expression status of proteins in vimentin knockdown as compared to vector control clone | |
|---|---|---|---|---|---|---|---|---|---|
| 1A | VIME_HUMAN (Vimentin, OS = Homo sapiens) | 53676 | 4.9 | 74 | 8 | 39 | 23.6% | Downregulated | |
| 2A | Keratin 14 | 51872 | 5.09 | 52 | 10 | 50 | 23% | Downregulated | |
| 3B | K1C17_HUMAN (Keratin Type 1, Cytoskeletal 17, OS = Homo sapiens) | 48361 | 4.8 | 156 | 15 | 26 | 50.9% | Unchanged | |
| 4A | Unidentified | Downregulated | |||||||
| 5A | K1C18_HUMAN (Keratin, Type I Cytoskeletal 18 OS = Homo sapiens) | 48029 | 5.2 | 76 | 17 | 60 | 51.4% | Unchanged | |
| 6A | K2C8_HUMAN (Keratin Type 2, Cytoskeletal 8, OS = Homo sapiens) | 53671 | Predicted as K8 but with low score | Unchanged | |||||
| 7A | ACTB_HUMAN (Actin, Cytoplasmic 1 and 2, OS = Homo sapiens) | 42052 | 5.2 | 75 | 7 | 57 | 38.9% | Unchanged | |
MALDI analysis: The resulting data of the keratin spots was analyzed using Flex analysis 3.0 (BruckerDaltonik, Germany) software. The peak list was searched against SwissProt database using MASCOT search engine. The last column shows the expression status of proteins in vimentin knockdown as compared to its vector control clone after validating the spots identified by MALDI, with western blotting.