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. Author manuscript; available in PMC: 2017 Feb 22.
Published in final edited form as: J Genet Syndr Gene Ther. 2016 Nov 14;7(5):311. doi: 10.4172/2157-7412.1000311

Figure 2.

Figure 2

(a) Representative agarose gel showing amplifications of ABCA4 transcripts isolated from fAAV (5, 6 and 7 days post-transduction left to right) and OV AAV (5 days post-transduction) treated HEK293T cells. (b) Representative western blot detection of ABCA4 (ab72955, Abcam) from HEK293T cells transduced with fAAV and OV AAV variants. Cells were transduced with a MOI of 10,000: x1, x2, x5, x10 & x20 indicate multiples of the MOI. ABCA4 CDS = ATP-binding cassette transporter protein member 4 coding sequence; WPRE = Woodchuck hepatitis virus post-transcriptional regulatory element; - = untransduced HEK293T; + = pABCA4 transfected HEK293T; fAAV = fragmented AAV in various serotypes; OV = overlapping dual vector AAV2/8Y733F; OV overlap = region of overlap contained in upstream and downstream transgenes that form the OV system.