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. Author manuscript; available in PMC: 2017 Feb 22.
Published in final edited form as: J Genet Syndr Gene Ther. 2016 Nov 14;7(5):311. doi: 10.4172/2157-7412.1000311

Figure 5.

Figure 5

PCR amplifications of ABCA4 transcripts in cDNA from transduced HEK293T cells with depictions of the transcripts identified following sequencing. Both fAAV8Y733F and OV AAV8Y733F treated samples contained correctly formed full length ABCA4 transcripts. For fAAV8Y733F treated samples, hybrid transcript sequences were identified for which upstream and downstream portions of ABCA4 CDS were fused within exons. The exons within which the hybrids were joined are shown for transcript variants A, B and C. UTC = untransduced HEK293T; fAAV = fragmented AAV2/8Y733F treated HEK293T; OV = overlapping dual vector AAV2/8Y733F treated HEK293T; + = ABCA4 transfected HEK293T; AAV2 genome = genetic material from the AAV2 genome containing major coat protein A intron.