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. Author manuscript; available in PMC: 2017 Feb 22.
Published in final edited form as: Curr Opin Chem Biol. 2013 Aug 9;17(5):738–746. doi: 10.1016/j.cbpa.2013.07.021

Figure 3.

Figure 3

FUNCAT labeling in dissociated neurons, organotypic hippocampal slices and whole mount zebrafish larvae. (a)–(d) Antibody and FUNCAT labeling of dissociated hippocampal neurons. Scale bar = 10 μm. (e)–(h) Antibody and FUNCAT labeling of organotypic hippocampal slice. FUNCAT labeling was achieved by incubating slice with AHA, ‘clicking’ to biotin alkyne and subsequent immunostaining with mouse anti-biotin primary antibody and Alexa Fluor-594 anti-mouse secondary antibody. Scale bar = 20 μM. (i)–(k) FUNCAT labeling of whole-mount 7-day-old larval zebrafish. (i) dorsal view; (j) lateral view. Scale bar = 150 μm. (k) Dorsal view of cross-section of tail, showing muscles and spinal cord. Scale bar = 20 μm. (l)–(n) Antibody and FUNCAT labeling of whole-mount 7-day-old larval zebrafish. Scale bar = 100 μm.