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. Author manuscript; available in PMC: 2017 Feb 22.
Published in final edited form as: Cancer Res. 2013 Oct 14;73(23):6913–6925. doi: 10.1158/0008-5472.CAN-13-0424

Figure 5.

Figure 5

Lentiviral-mediated knock down of PIP4K2B leads to a decrease in CDH1 expression. A. MCF7 cells transduced with control (1) or with shRNA constructs targeting PIP4K2B (2 and 3) were fixed and immunostained with antibodies to CDH1 and analysed by fluorescence microscopy. Total immunofluorescence staining was determined and is graphically presented. Statistically, differences were determined using Students t-test between control and PIP4K2B knockdown cells (** P <0.01). B. MCF10A cells were transduced with control (pLKO1) or with lentiviral-mediated shRNAs against PIP4K2B (#1 and #2). Cell lysates were immunoprobed with the indicated antibodies. C. Control and PIP4K2B knockdown MCF10A cells were with MG132 as indicated. Cell lysates were western blotted with the indicated antibodies. D. Control and PIP4K2B knockdown MCF10A cells were assessed for the relative expression of PIP4K2B and CDH1 mRNA using qRT-PCR. The data are presented as fold change compared to control. E. MCF10A cells transduced with the indicated shRNAs were transfected with Renilla and E-cadherin-luc. A luciferase activity was measured and the data are presented after correction for the expression of the control Renilla luciferase.