Fig. 5.

(A) Induction of peptide- and rAlt a 1- specific IgG antibodies in rabbits. Rabbits were immunized with KLH-coupled Alt a 1-derived peptides or rAlt a 1. IgG reactivity of the final sera to ELISA plate bound rAlt a 1 was determined for different dilutions (x-axis) of the anti-sera and is displayed as optical density (OD) values (y-axis). (B) Anti-peptide anti-sera and anti-rAlt a 1 anti-serum inhibit Alternaria-sensitized patients’ IgE binding to rAlt a 1. Pre-incubation of ELISA plate bound rAlt a 1 with rabbit anti-peptide anti-sera, mixtures of equal amounts of anti-peptide anti-sera or anti-rAlt a 1 anti-serum reduces patients’ IgE binding (n = 38) to rAlt a 1. The percentage inhibition of IgE binding was calculated and is represented in box plots, where boxes mark the interquartile range containing 50% of the data, and lines across the boxes indicate the median. ○ represent outliers.