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. Author manuscript; available in PMC: 2017 Feb 22.
Published in final edited form as: Bio Protoc. 2016 Dec 5;6(23):e2056. doi: 10.21769/BioProtoc.2056

Video 2. Motility of LBPs in Dictyostelium.

Video 2

50 μl of cell suspension after the chase step was placed on a clean glass coverslip, allowed to adhere to the surface for 3-5 min and then imaged under 100x objective of a DIC microscope. The movie was acquired at 30 frames/sec using a custom program written on LabVIEW and later reduced to 10 frames/sec. Movie runs in real time. Latex beads (now LBPs), being highly refractile, can be easily distinguished from other endogenous organelles and show robust motion in vivo.