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. Author manuscript; available in PMC: 2017 Feb 22.
Published in final edited form as: Proteomics Clin Appl. 2012 Jun;6(5-6):244–256. doi: 10.1002/prca.201100042

Table 3.

Glycoproteomics in lung cancers.

Investigators Samples Methods Major Findings
Soltermann
A, et al, 2008
[72]		 Pleural effusion
of 5 adenoca
(advance stage)
lung cancer cell
line A549		 Hydrazide
enrichment and
LC-MS/MS

  • 6 Glycoproteins were identified and correlated with tumor tissue by IHC.

  • Periostin was identified in pleural effusion.
Hongsachart
P, et al, 2009
[81]		 Sera of 10
adenoca (stage
II/III)		 Con A, WGA
and others
lectin-affinity
2-D PAGE, 2-D
DIGE
MALDI-TOF
MS, MS/MS

  • 27 glycoproteins were up-regulated and 12 were down-regulated.

  • Among them, 3 up- and 2 down-regulated glycoprotein were validated by Western blotting.
Rho JH, et al,
2009 [65]		 Fresh tumor
tissue of 16
adenoca (stage
II/III)		 Glycoarray
2-D PAGE
LC-MS/MS

  • 8 glycoproteins were up- and 7 were down-regulated.

  • Differential glycosolation pattern was identified between cancer and matched normal tissues.
Rho JH, et al,
2009 [64]		 Fresh tumor
tissue of 5
adenoca (stage
II/III)		 2-D DIGE
LC-MS/MS

  • 14 spots were differentially expressed.

  • 3 proteins were overexpressed by LC-MS/MS.

  • Differential protein expression pattern was identified between cancer cells and stroma.
Ueda K, et al,
2009 [83]		 Sera of 10
adenoca (stage
IV)		 SELDI-TOF
MS
Lectin-coupled
ProteinChip
array

  • 41 protein peaks showed significant differences.

  • Cancer-associated aberrant glycosylations were identified
Ueda K, et al,
2010 [82]		 Sera of 8
adenoca* 		Con A lectin-
chromatography
Combination of
IGEL with 8-
plex iTRAQ

  • 107 glycoproteins were identified.

  • 8 glycoproteins showed significant differences.
Zeng X, et al,
2010 [80]		 Pooled sera of 31
adenoca* and 23
SqCC* 		Hydrazide
enrichment and
LC-MS/MS

  • 22 glycoproteins showed significantly differential expressions

  • Three of them were verified by ELISAs.
Tsai HY, et
al, 2011 [84]		 Sera of 19
adenoca*, 8
SqCC*, 11
SCLC*, and 7
unknown types* 		2-D DIEG
MALDI
MS/MS
Nanospray-MSn

  • Fucosylated haptoglobin was elevated in all cancers.
*

tumor stage is not specified. Adenoca: adenocarcinoma. SqCC: squamous cell carcinoma. SCLC: small cell lung cancer.