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. Author manuscript; available in PMC: 2017 Feb 22.
Published in final edited form as: Methods Mol Biol. 2015;1300:123–139. doi: 10.1007/978-1-4939-2596-4_9

Fig. 3.

Fig. 3

(a) Schematic diagram of the orientation of the URA3 reporter gene on chromosome III adjacent to the ARS306 origin of replication. Template strands are in black, the nascent leading strand is in blue and the nascent lagging strand in green. The orientation of the reporter gene with respect to coding sequence is indicated as orientation 1 (OR1) or orientation 2 (OR2). Strand-specific radiolabeled probes that anneal to one of the two nascent strands are designated Probe A and Probe B. Their strand-specificity is dependent on the orientation of URA3. (b) An example of a southern blot probing for alkali-sensitive sites in the nascent leading and lagging strands of yeast genomic DNA. All strains in this experiment harbor the pol2-M644G variant that has increased capacity to incorporate ribonucleotides [8, 12, 13]