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. Author manuscript; available in PMC: 2017 Jul 23.
Published in final edited form as: Nat Immunol. 2017 Jan 23;18(3):354–363. doi: 10.1038/ni.3665

Fig. 8. The sMAC is formed in mouse T cells.

Fig. 8

(a) Immunoblot analysis and (b) pooled data (n = 4) of Erk, Jnk, and p38 phosphorylation in lysates from CD4+ T cells from 20-month old vaccinated Sesn1+/- and Sesn1-/- mice following immunoprecipitation with sestrin2 antibodies. Results in (b) presented relative to those of Sesn1+/- mice, set as 1. Sestrin2 expression in whole cell lysates (WCL), loading control. Sestrin1 expression is also shown. (c) Image-Stream analysis and (d) % co-localization scores of sestrin2+-pMAPKs+ in CD4+ T cells from Sesn1-/- and Sesn1-/+ mice, five days post-vaccination with FLUAD, presented as in (b). In vitro MAPK assays of sestrin2 immunoprecipitates from mouse Sesn1-/- CD4+ T cells incubated for 60’ with or without (e) recombinant human sestrin1 or (f) the AMPK agonist A-769662, in the presence or absence of ATP. Results presented relative to that of non-reconstituted Sesn1-/- cells (‘basal’), set as 1. In (e) cells were transfected with the indicated siRNAs 48h before lysis. Data are representative of four experiments (a, c) or pooled from four experiments with four (age-matched) individual mice (b, d, e, f). **p<0.01 and ***p< 0.001, ANOVA for repeated measures with Bonferroni post-test correction. Error bars indicate s.e.m. throughout.