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. 2016 Sep 18;72(3):416–424. doi: 10.1111/all.12992

Figure 1.

Figure 1

(A) Detection of proteins in milk formulas M1–M10 by SDS‐PAGE and Coomassie Brilliant Blue Staining. Aliquots of each milk formula (lanes M1–M10) were loaded. Lanes M show molecular weight markers (kDa). (B) Reactivity of M1–10 with specific antibody probes. Aliquots of the samples were dotted in duplicates onto nitrocellulose and incubated with rabbit antibodies raised against recombinant cow's milk (CM) proteins (rαS1‐cas, rαS2‐cas, rβ‐cas, rκ‐cas, rα‐la, rβ‐lg, rlf), with normal rabbit serum (nrs) or with buffer alone.