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. Author manuscript; available in PMC: 2017 Feb 22.
Published in final edited form as: Process Biochem. 2015 Jul;50(7):1158–1166. doi: 10.1016/j.procbio.2015.03.020

Table 2.

Secondary structure analysis (from FTIR spectra) for BSA in the studied protein stabilizers at room temperature and pH = 7.4.

Protein stabilizers Concentration Amide I components / (%)
inter β-sheet intra β-sheet α-helix turn antiparallel β-sheet
Tricine 0.05 M[42] 2.4[42] 25.0[42] 57.6[42] 14.2[42] 0.8[42]
0.5 M ― 24.3 58.3 17.4 ―
TES 0.05 M ― 24.3 59.8 15.9 ―
0.5 M ― 29.1 61.2   9.7 ―
HEPES 0.05 M ― 26.4 58.3 25.3 ―
0.5 M 3.0 20.9 59.7 16.4 ―
sucrose 0.05 M ― 30.0 59.9 10.1 ―
0.5 M ― 24.8 61.5 13.7 ―
[Ch]Cl 0.05 M ― 25.4 59.5 15.1 ―
0.5 M ― 25.3 60.0 14.7 ―
[Ch][Tricine] 0.05 M ― 25.6 61.1 13.3 ―
0.5 M ― 27.3 62.9   9.8 ―
[Ch][TES] 0.05 M ― 29.7 62.5   7.8 ―
0.5 M ― 25.7 63.1 11.2 ―
[Ch][HEPES] 0.05 M ― 27.5 60.0 12.5 ―
0.5 M ― 29.9 61.1   9.0 ―