Table 1.

Monoclonal antibody binding affinity values for polymyxin lipopeptides.

Polymyxin lipopeptide	Polymyxin lipopeptide sequence	KD (µM)
Colistin	Octanoyl-Dab-Thr-Dab-Dab*-Dab-D-Leu-Leu-Dab-Dab-Thr*	0.56
Polymyxin B	Octanoyl-Dab-Thr-Dab-Dab*-Dab-D-Phe-Leu-Dab-Dab-Thr*	0.26
FADDI-019	Octanoyl-Dab-Thr-Dab-Dab*-Dab-D-OctGly-Leu-Dab-Dab-Thr*	0.43
FADDI-020	4-Biphenylcarboxyl-Dab-Thr-Dab-Dab*-Dab-D-OctGly-Leu-Dab-Dab-Thr*	0.94
FADDI-043	Dansylgly-OctGly-Dab-Thr-Dab-Dab*-Dab-D-Phe-Leu-Dab-Dab-Thr*	2.71
FADDI-063	Octanoyl-Dab-Thr-Dab-Dab*-Dab-D-Phe-Cys(Bz)-Dab-Dab-Thr*	5.15
FADDI-187	4-tert-Butylphenylacetyl-D-Dab-Dab*-Dab-D-Phe-Leu-Dab-Dab-Thr*	5.39
FADDI-053	Dansylgly-Dab-Thr-Dab-Dab*-Dab-D-Phe-Leu-Dab-Dab-Thr*	5.50
FADDI-206	Octanoyl-Dab-Thr-Dab-Dab*-Dab-D-Phe-Met-Dab-Dab-Thr*	8.80
FADDI-065	Octanoyl-Dab-Thr-Lys-Dab*-Dab-D-Phe-Leu-Dab-Dab-Thr*	13.1
FADDI-207	Octanoyl-Dab-Thr-Dab-Dab*-Dab-D-Met-Leu-Dab-Dab-Thr*	13.4
FADDI-180	Octanoyl-Dab-Thr-Pro-Dab*-Dab-D-Phe-Leu-Dab-Dab-Thr*	20.3
FADDI-167	Octanoyl-Dab-Thr-Dab-Dab*-Dab-D-Phe-Leu-Ala-Dab-Thr*	NSB
FADDI-168	Octanoyl-Dab-Thr-Dab-Dab*-Dab-D-Phe-Leu-Asn-Dab-Thr*	NSB
FADDI-169	Octanoyl-Dab-Thr-Dab-Dab*-Dab-D-Phe-Leu-Thr-Dab-Thr*	NSB
FADDI-170	Octanoyl-Dab-Thr-Dab-Dab*-Dab-D-Phe-Leu-Dab-Ala-Thr*	NSB
FADDI-171	Octanoyl-Dab-Thr-Dab-Dab*-Dab-D-Phe-Leu-Dab-Asn-Thr*	NSB
FADDI-172	Octanoyl-Dab-Thr-Dab-Dab*-Dab-D-Phe-Leu-Dab-Thr-Thr*	NSB
FADDI-175	Octanoyl-Dab-Thr-Dab-Dab*-Ala-D-Phe-Leu-Dab-Dab-Thr*	NSB
FADDI-176	Octanoyl-Dab-Thr-Dab-Dab*-Asn-D-Phe-Leu-Dab-Dab-Thr*	NSB
FADDI-177	Octanoyl-Dab-Thr-Dab-Dab*-Thr-D-Phe-Leu-Dab-Dab-Thr*	NSB
Colistin nonapeptide	Thr-Dab-Dab*-Dab-D-Leu-Leu-Dab-Dab-Thr*	NSB

NSB. No significant binding (K_D>25 µM)

^*Cyclisation points