Fig. 6.

LCMV modulates expression of functionally important surface molecules on mature DCs. At day 15 after infection, spleens from ARM- or Cl 13-infected mice were collected and individual splenocyte suspensions were prepared from each mouse. Splenocytes were stained with specific monoclonal antibodies for CD11c, MHC II, MHC I, CD80, and CD86. The figure shows FACS histograms of surface expression of MHC I, MHC II, CD80, and CD86 on CD11c⁺ cells. The gray filled-in curves represent expression of the corresponding marker molecules on DCs from Cl 13-infected mice, whereas the black curves show surface expression on DCs from ARM-infected mice. Broken lines depict staining of DCs with an irrelevant antibody (isotype control). In the X-axis, the fluorescence intensity (log scale) is given, whereas the Y-axis depicts the relative cell number