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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Dec 17;1860(3):588–598. doi: 10.1016/j.bbagen.2015.12.012

Figure 1. Expression of Syk and its influences on the maturation of dendritic cells.

Figure 1

(A)Western blot of the whole cell lysates from wild type and Cre-loxP-mediated Syk deficient dendritic cells. Blots were probed with antibodies to total-Syk and GAPDH as a loading control; (B) Densitometric quantification of the ratio of total-Syk to total-GAPDH; (C) WT and Syk deficient cells were stimulated with LPS (1 μg/ml) for 24 hours, and the expression of CD80 and CD86 was detected by flow cytometry; (D) Cell viability was determined by trypan blue exclusion.