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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Dec 17;1860(3):588–598. doi: 10.1016/j.bbagen.2015.12.012

Figure 5. Inhibition of PI3K or Akt abrogates the regulatory effects of Syk deficiency on TLR4-mediated IFNβ and IL-10 production.

Figure 5

(A, B) Syk deficiency cells were pre-treated with LY294002 (25 μM) or ZSTK474 (50 nM) and stimulated with LPS (1 μg/ml). Cell lysates were collected at the time points indicated and phosphorylation of Akt were detected by Western blot. (C, D) Densitometric quantification of the ratio of phospho-Akt to total-GAPDH. (E, F) Wild type and Syk deficient cells were pretreated with PI3K inhibitor, LY294002 or ZSTK474, or Akt inhibitor, for 2 h and then stimulated with LPS for 24 h. Cell free supernatants were collected and LPS-induced production of IFNβ (E) and IL-10 (F) was determined by ELISA. “***” indicates P<0.001. Data represent the mean ± S.D. of three biological replicates.