Figure 3. Effect of compound 1o on P. berghei multiplication in mice and on parasite transmission to mosquitoes.

(a) Naive mice were injected i.v. with infected blood and treated daily for 3 days starting 24 h post passage with compound 1o (100 mg kg⁻¹), with methylene blue (MB, 15 mg/kg), or with vehicle (Tw80) as positive and negative controls, respectively. Parasitemia was monitored daily and the mean± s.d. of three independent experiments are plotted. Groups of mosquitoes were fed on mice 24 h after each treatment. (b) Prevalence (percentage of infected mosquitoes) and (c) infection intensity (mean number of parasites per infected midgut) were determined 7 days post infectious blood feeding. The results of four independent experiments were pooled, and the mean±s.e. of the mean is shown (b,c). Significance for differences between treatments was calculated using generalized linear models with treatment, time, and repeats as variables, ***P value <0.001 (a–c). n, number of mosquitoes. (d) Effects of compound 1o on early, mid, and late stage P. falciparum gametocytes. Representative data sets are given (4.5% gametocytaemia) out of three independent experiments. Survival of treated gametocytes was calculated for each concentration relative to parasites exposed to vehicle.