Skip to main content
. 2017 Feb 17;8:14487. doi: 10.1038/ncomms14487

Figure 8. Transient Schwann cell ablation causes permanent disruption of the heminodes and dysmyelination at the HP.

Figure 8

(a–f) Heminodes at the habenula perforata (HP) remain disorganized in DTA(+/−):Plp1/Cre(+/−) cochlea (b,d,f) compared control cochlea (a,c,e) at 1 week (a,c), 4 weeks (b,d) and 16 weeks (e,f) after tamoxifen induction. (g) Increased number of aberrant nodal staining (co-localized Caspr/Gliomedin) is observed in DTA(+/−):Plp1/Cre(+/−) cochlea outside the pre-defined 20 μm heminode zone as shown in a–f (below dashed lines, n=6–10 of each animal group). ***P<0.001 by two-way ANOVA followed by Bonferroni's post test. Data are expressed as mean±s.d. (h,i) Immunostaining for NFH, which preferentially stains unmyelinated axons, indicates the presence of dysmyelinated ANFs as they reach the HP (i, arrow heads) 16 weeks after tamoxifen induction in DTA(+/−):Plp1/Cre(+/−) cochlea. This staining is absent in control mice (h). HP, habenula perforata; IHC, inner hair cells; OSL, osseous spiral lamina. IHCs are stained for Myo7a. (j,k) Representative electron micrographs show dysmyelinated nerve fibres beneath the HP in DTA(+/−):Plp1/Cre(+/−) cochlea (k) but not in control cochlea (j).