Figure 2.

Etv5 deficient Th2 cells produce less IL-10 in Aspergillus fumigatus extract-induced airway inflammation. A, Control and Etv5^fl/fl CD4 Cre⁺ mice were intranasally challenged with A.fumigatus extract every other day for 21days. B-D, Total cell count (B), eosinophils (C) in the BAL and total cell count in the lung (D) of A.fumigatus extract-challenged control and Etv5^fl/fl CD4 Cre⁺ mice. E-F, Lung and BAL cells were stimulated with PMA/Ionomycin for 6 hours to measure cytokine production using intracellular staining. Representative dot plots (E) and average percentage of IL-10 producing Th2 cells in BAL and lung (F) are indicated. Cells for flow cytometric analysis were gated on lymphocyte size and granularity, and expression of CD4 and TCRβ. G, IL-10 production of BAL cells was measured using ELISA. Data are mean ± SEM of 2 independent experiments (n=6/experiment). *p < 0.05, **p <0.005