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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: J Immunol. 2017 Jan 27;198(5):2156–2164. doi: 10.4049/jimmunol.1601757

Figure 5. Potassium efflux is required for the release of bioactive IL-1β but not for cell death upon MLKL activation.

Figure 5

A-B. Cell death kinetics of THP-1 cells stably expressing acMLKL in response to LPS + nigericin (A) or MLKL activation (B) were tracked using an IncuCyte bioimager. C. Western blot analysis of THP-1 cell supernatants harvested five hours after addition of nigericin or AP1. D. IL-1β bioassay of THP-1 cells expressing acMLKL, treated as indicated. Bioactive IL-1β in the supernatants was measured five hours post treatment and reported as (Units/ml). For each experiment three replicates for each experimental condition were assayed. Standard curve for each experiment was constructed using recombinant human IL-1β and used to calculate and report Units/ml of IL-1β. Error bars represent S.D. from the mean of a minimum of three independent wells. Statistical significance was calculated by student's t-test using GraphPad Prism software. Each result depicted is representative of at least three independent experiments. *p< 0.05