Figure 4.
CLSM of S. epidermidis O-47 and S. epidermidis RP62A biofilms in the presence and absence of pentadecanal. (A) CLSM of S. epidermidis O-47 and S. epidermidis RP62A biofilms in BHI medium without pentadecanal and (C) with pentadecanal 100 μg/ml. The bacteria were grown in chamber slides for 20 h and then stained with LIVE/DEAD reagents. The green fluorescence (SYTO9) indicates viable cells PI and the red fluorescence (PI) indicates dead cells. (B) Z-stack analysis of S. epidermidis O-47 and RP62A biofilms without pentadecanal. (D) Z-stack analysis of S. epidermidis O-47 and RP62A biofilms treated with 100 μg/ml pentadecanal.