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. 2017 Feb 23;7:53. doi: 10.3389/fcimb.2017.00053

Figure 1.

Figure 1

AFB1 induced a time- and dose-dependent autophagic response. (A) RAW264.7 cells were transfected with GFP-LC3 plasmid for 12 h. The cells were pretreated with Rapa (5 μM, 12 h) and wortmannin (100 nM, 1 h) and then treated with AFB1 (0.25 μM) for 2 h. Scale bars = 20 μm. (B) The percentage of GFP-LC3 puncta cells was calculated. ***P < 0.001 compared with the control groups; ###P < 0.001 compared with Rapa and AFB1. (C,D) RAW264.7 cells and THP-1 cells were pretreated with 5 μM Rapa for 12 h and then treated with 0.25 μM AFB1 for different times. (G,H) The two cell lines were similarly treated with Rapa and subsequently exposed to different concentrations of AFB1 for 1.5 h. (E,F, I,J) Western blotting was conducted to assay the level of LC3. The ratio of LC3-II/GAPDH was calculated. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the control groups in the same cell line. The data are representative of three experiments with similar results.