Figure 4.
AFB1 induced the generation of METs. (A) THP-1 cells were incubated in 24-well glass-bottom plates (2 × 105 cells/well) with serum-free RPMI 1640 for 12 h. Cells were treated with PMA (16 nM) and AFB1 (2 μM) at concentrations of 0.03, 0.12, 0.5, or 2 μM for 2 h. METs were stained with SYTOX Orange (5 mM) for 10 min, and nuclei were stained with Hoechst 33342 (1 μM) for 5 min. (B) Histone, elastin, and eDNA colocalized. (C) Histone, myeloperoxidase, and eDNA colocalized. The images were obtained by fluorescence microscopy with a 20 × objective lens. Scale bars = 50 μm.