Figure 7.

METs formation reduced the AFB1 content. (A) RAW264.7 cells were incubated in 24-well plates (2 × 10⁵ cells/well) with serum-free medium for 12 h. Only one of the wells was pretreated with DPI (50 μM) for 1 h, and then CytD was added to all of wells for 20 min, followed by treatment with AFB1 (2 μM) or PMA (16 nM) and AFB1 (2 μM) for 8 h. We used the AFB1 ELISA to determine the AFB1 content through an ELISA kit. The cells were treated with AFB1 with or without PMA, or AFB1 with or without DPI. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 compared with the control groups (only cells) in the same cell line. The data are representative of three experiments with similar results. (B) The fluorescence intensity of Extracellular DNA was detected by a plate reader. The data are representative of three experiments with similar results.