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. 2017 Feb 23;7:53. doi: 10.3389/fcimb.2017.00053

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Copyright © 2017 An, Shi, Tang, Wang, Shen, Zhang, Wang, Jiang, Liu and Yu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AFB1 induced M1-like polarization in RAW264.7 cells. (A) The expression levels of CD86 and CD206 in RAW264.7 cells were detected by flow cytometry. The results are presented as the mean fluorescence intensity (MFI). ^***P < 0.001 compared with the control groups of RAW264.7 cells. The data are representative of three experiments with similar results. The histogram figure represents the comparison of the 2 μM AFB1-treated group and control group. (B) CD86 and CD206 in RAW264.7 cells, Atg7-silenced cells and cells transfected with the shRNA negative control were detected by flow cytometry. The results are presented as the mean fluorescence intensity (MFI). ^***P < 0.001 compared with the control groups of RAW264.7 cells; ^###P < 0.001 compared with AFB1-infected cells and cells transfected with the negative control shRNA plasmid. The histogram figure represents the comparison of the 2 μM AFB1-treated group, Atg7-silenced cells, cells transfected with the shRNA negative control and control group. (C) iNOS, Arg-1 and eDNA colocalized, and the immunofluorescence staining shows the comparison of the 2 μM AFB1-treated Non-transfected cell group, Atg7-silenced cell group, cells transfected with the shRNA negative control group and control group. (D) TNF-α and IL-10 secretion was measured by ELISA. ^***P < 0.001 compared with the control group; ^###P < 0.001 compared with the AFB1-infected cells and cells transfected with the negative control shRNA plasmid.