Skip to main content
. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: J Immunol. 2017 Jan 25;198(5):1974–1984. doi: 10.4049/jimmunol.1600797

Figure 4. Knockdown of TLR7 and TLR8 affects HCV-mediated monocyte differentiation and macrophage polarization.

Figure 4

Monocytes were treated with Huh7.5 or Huh7.5/JFH1 concentrate or were left untreated. After 7 days, the total RNA was isolated and cDNA synthesized. PCR was performed for TLR3, TLR7 and TLR8. (A) The graphs show the mRNA levels of TLR3, TLR7 and TLR8. (B–D) Monocytes were transfected with scrambled or TLR3, TLR7 or TLR8 siRNA for 48 hours and the transfected monocytes were stimulated for 5 days with Huh7.5 or Huh7.5/JFH1 concentrate and (B) cell surface expression of CD68, CD16 and CD206 were performed by flow cytometry. (C–D) Cell culture supernatant collected was assayed for TNFα, IL-1β, IL-10 and TGFβ by ELISA. The data is shown as mean±SEM, n=3–5, *P ≤ 0.05.