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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: J Immunol. 2017 Jan 16;198(5):1985–1994. doi: 10.4049/jimmunol.1502609

Figure 6. Burkholderia species further reduce ROS production in CF MDMs.

Figure 6

A) CF MDMs have reduced ROS production as measured by the DCF assay in response to 30 min infection with Burkholderia cenocepacia (Bc) and Burkholderia multivorans (Bm) compared to non-CF, representative image of n=6. B) Summed end-point analysis of 5A experiments, results are expressed as a percentage of non-CF bacteria-stimulated MDMs for six independent experiments. Bc P value =0.005, Bm P value =0.008, n=6. C) CF MDMs have reduced phosphorylation of p40phox and p47phox during Bc infection. Western blots of cell lysates for phosphorylated and total NADPH components after 30 min infection with Bc. Representative image of > 3 experiments. D) Densitometric analysis of 3 Western blots per condition in 5C, normalized to the loading control calreticulin.