Table 1.
T cell proliferative responses of Apoe−/− mice to apolipoprotein B-100 (ApoB-100) peptides.
| Immunization with ApoB-100 peptides | T cell proliferative responses [counts per minute and stimulation index (SI)]a |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Medium | Peptides for in vitro stimulation (μg/ml) |
||||||||
| P1 (50) | P2 (50) | P3 (50) | P4 (50) | P5 (50) | P6 (50) | Purified protein derivative (PPD) (20) | Con A (1) | ||
| P1 (mApoB-1003066–3080) | 2631 ± 187 | 3,524 ± 209 (0.34) | 3,904 ± 341 (0.48) | 4,791 ± 528 (0.82) | 4,763 ± 406 (0.81) | 3,136 ± 255 (0.19) | 2,508 ± 341 (−0.06) | 19,697 ± 1,571 (6.49) | 15,603 ± 2,089 (4.93) |
| P2 (mApoB-100438–452) | 1,998 ± 164 | 1,807 ± 365 (−0.10) | 1,244 ± 173 (−0.38) | 2,689 ± 404 (0.35) | 4,197 ± 273 (1.10) | 3,411 ± 557 (0.71) | 3,049 ± 586 (0.53) | 15,486 ± 1,623 (6.79) | 24,273 ± 2,907 (11.15) |
| P3 (mApoB-1003501–3515) | 1,728 ± 226 | 2,447 ± 253 (0.42) | 1,695 ± 208 (−0.02) | 13,064 ± 1,507 (6.56) | 1,918 ± 202 (0.11) | 1,594 ± 183 (−0.09) | 2,067 ± 204 (0.20) | 10,981 ± 1,334 (5.35) | 15,543 ± 1,828 (7.99) |
| P4 (ApoB-1001578–1592) | 3,039 ± 415 | 2,188 ± 407 (−0.28) | 4,637 ± 298 (0.53) | 1,906 ± 204 (−0.12) | 2,385 ± 175 (−0.22) | 3,563 ± 420 (0.17) | 2,917 ± 483 (−0.04) | 20,089 ± 1,472 (5.61) | 18,146 ± 1,469 (4.97) |
| P5 (ApoB-1004054–4068) | 1,386 ± 277 | 2,845 ± 639 (1.05) | 3,247 ± 303 (1.34) | 2,055 ± 148 (0.48) | 2,162 ± 257 (0.60) | 1,849 ± 103 (0.33) | 2,408 ± 376 (0.74) | 18,924 ± 2,681 (12.65) | 16,901 ± 2,011 (11.19) |
| P6 (ApoB-100978–992) | 3,236 ± 496 | 4,805 ± 611 (0.49) | 2,735 ± 591 (−0.15) | 3,804 ± 681 (0.18) | 4,539 ± 509 (0.40) | 5,001 ± 786 (0.55) | 28,723 ± 4,162 (7.88) | 22,508 ± 2,937 (5.96) | 25,924 ± 2,062 (7.01) |
Separate groups of Apoe−/− mice were immunized in four sites in the flanks with each of the ApoB-100 peptides emulsified in complete Freund’s adjuvant. Ten days later, popliteal and axillary lymph node cells were isolated. The cells were cultured separately with or without 50 μg/ml of each of the peptides for 4 days. PPD and concanavalin A (Con A) were included as positive control. Sixteen hours before the end of the culture period, 1 μCi of tritiated thymidine (3H) was added to each well. Cells were harvested on an automatic cell harvester and incorporation of 3H was determined in a Beckman scintillation counter. All cultures were established in triplicates, and the results were expressed as the mean number of counts per minute ± SEM.
aSIs are calculated by the following formula:
Bold font indicates the important data.