Table 2.

CD4⁺ and CD8⁺ T cell subsets responding to peptides 3 and 6.

			In vitro proliferative responses (stimulation index)a
Immunizing Ag (200 μg/mouse)	Cell populations (2 × 105 cells)	Medium (cpm)	Peptide3 (100 μg/ml)	Peptide6 (100 μg/ml)	MOG35–55 (100 μg/ml)
Peptide 3	Unseparated	2,387	18.0 ± 4.6	0.0 ± 0.5	−1.0 ± 0.2
	CD4+	1,911	24.0 ± 6.0	0.0 ± 0.3	−1.0 ± 0.1
	CD8+	1,764	−1.0 ± 0.0	−0.1 ± 0.0	−1.0 ± 0.3
Peptide 6	Unseparated	2,005	0.0 ± 0.4	9.0 ± 0.8	−1.0 ± 0.2
	CD4+	1,793	0.0 ± 0.4	22.0 ± 3.2	−1.0 ± 0.1
	CD8+	1,862	0.0 ± 1.9	0.0 ± 0.3	2.0 ± 0.5

Apoe^−/− mice were immunized with P3 or P6 as described in Table 1. Ten days later, draining lymph node cells from both groups were isolated. The cells were passed through anti-CD4 microbead MAC columns and anti-CD8 columns from Miltenyi Biotech to positively isolate CD4⁺ and CD8⁺ T cells, respectively. Purified cells, together with unseparated cells were assayed for T cell proliferative responses. All cultures were established in triplicates, and the results were expressed as the mean number of counts per minute ± SEM.

^aSIs are calculated by the following formula: $\frac{\mathit{counts\; per\; minutes\; (CPM)\; experimental}-\mathit{counts\; per\; minute\; (CPM)\hspace{0.17em}medium\; background}}{\mathit{counts\; per\; minute\; (CPM)\; medium\; background}}.$

Bold font indicates the important data.