Table 3.

T cell proliferative responses of human apolipoprotein B-100 (hApoB-100)-primed lymph node cells.

		In vitro proliferative responses [stimulation index (SI)]a
Immunizing Ag (100 μg/mouse)	Medium (cpm)	hApoB-100 (50 μg/ml)	P6 (50 μg/ml)	Purified protein derivative (PPD) (20 μg/ml)	OVA (100 μg/ml)
hApoB-100	3,354	3.30	4.03	8.35	0.04
P6	3,567	2.86	7.25	8.87	0.15

Groups of Apoe^−/− mice were immunized with hApoB-100 native molecule and P6 following the procedures described in Table 1. T cell proliferative assay was performed 10 days later. PPD was used as positive controls and OVA as negative controls. All cultures were established in triplicates.

^aSIs are calculated by the following formula: $\frac{\mathit{counts\; per\; minutes\; (CPM)\; experimental}-\mathit{counts\; per\; minute\; (CPM)\hspace{0.17em}medium\; background}}{\mathit{counts\; per\; minute\; (CPM)\; medium\; background}}.$