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. 2016 Mar 22;22(3):407–416. doi: 10.1038/mp.2016.23

Figure 3.

Figure 3

Osmotin (Os) reduced amyloid-β (Aβ) production in APPswe/ind-transfected SH-SY5Y cells in an AMPK/SIRT1/SREBP2-dependent manner. Western blot analysis and respective density histograms of (a) amyloid precursor protein (APP) and Aβ, (b) APP and (c, d) BACE1 and SREBP2 (sterol regulatory element-binding protein 2) in APPswe/ind-transfected SH-SY5Y cells subjected to the small interfering RNA (siRNA)-induced silencing of AMP-activated protein kinase (AMPK), sirtuin 1 (SIRT1) and SREBP2, respectively. Cells were transfected for 72 h followed by siRNA treatment for 48 h and osmotin treatment for 24 h. The β-actin signal is shown as a loading control. (e) Osmotin treatment increased the levels of sAPP-α, ADAM10 and ADAM17 protein expression in brain tissues homogenates from 9-month-old APP/presenilin 1 (PS1) mice compared with vehicle-treated mice. (f–h) ADAM10 western blot analyses and respective density histograms of APPswe/ind-transfected SH-SY5Y cells subjected to the siRNA-induced silencing of AMPK and SIRT1. All the respective details are described in the Materials and methods section. Significance: *P<0.05, **P<0.01 and ***P<0.001.