Figure 1.
Fluorescence in situ hybridization (FISH) and transmission electron microscopy (TEM) of ‘Ca. Desulfovibrio trichonymphae' phylotype Rs-N31 cells associated with a Trichonympha agilis cell. (a) Phase-contrast image of T. agilis. (b) FISH analysis using oligonucleotide probes specific to ‘Ca. Desulfovibrio trichonymphae' (6-carboxyfluorescein-labelled, green) and to ‘Ca. Endomicrobium trichonymphae' (Texas red-labelled, red), respectively (Sato et al., 2009). (c) TEM image of ‘Ca. Desulfovibrio trichonymphae' cells in a T. agilis cell. Pores opening to the outside of the T. agilis cell were observed (arrows). H, hydrogenosome. (d) Magnified image of a ‘Ca. Desulfovibrio trichonymphae' cell in panel c. IM, inner membrane; OM, outer membrane; HPM, host plasma membrane. Bars: 20 μm (a, b); 500 nm (c); 100 nm (d).