Figure 3.

(a) Specificity of ¹¹¹In-DOTA-3A3 binding to HER3-expressing BxPC-3 cells. Cells were incubated with 0.1 nM of ¹¹¹In-DOTA-3A3, for saturation of receptors 5 nM solution of non-labelled 3A3 was used. (b) Uptake and internalization under continuous incubation with 0.1 nM of ¹¹¹In-DOTA-3A3 with BxPC-3 cells at 37 °C. Data are presented as an average for 3 dishes ± SD. (c) Cell-associated radioactivity (cpm/10⁶ cells) during continuous incubation of BxPC-3 cells with ¹¹¹In-DOTA-3A3 at 37 °C with and without 20 mM sodium azide/10 mM 2-deoxyglucose. (d) Representative uptake/retention LigandTracer sensorgrams of ¹¹¹In-DOTA-3A3/HER3 interaction with BxPC-3 cells. Raw data (upper line) and data after subtraction of reference radioactivity (lower line).